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Course Details
KTO KARATAY UNIVERSITY
Graduate Education Institute
Programme of Sports Management Thesis Master's Degree
Course Details
Course Code Course Name Year Period Semester T+A+L Credit ECTS
84411121 Laboratory Methods in Muscle Physiology 2025 Autumn 1 3+0+0 3 7
Course Type Elective
Course Cycle -
Course Language Turkish
Methods and Techniques Students'performance is assessed based on their theoretical knowledge, experimental data analysis, literature/article appraisal, presentations or assignments, and midterm and/or final examinations.
Mode of Delivery Face to Face
Prerequisites There are no prerequisites for this course.
Coordinator -
Instructor(s) Asst. Prof. Elif GÜLBAHÇE MUTLU
Instructor Assistant(s) -
Course Instructor(s)
Name and Surname Room E-Mail Address Internal Meeting Hours
Asst. Prof. Elif GÜLBAHÇE MUTLU BBLOK-BZ02 [email protected] 7412 Monday
13:00
Course Content
Experimental foundations of muscle physiology; sampling and preparation of muscle tissue; isolated muscle preparations; measurement of contractile force and tension; assessment of isometric and isotonic contractions; electrical stimulation methods; single muscle twitch, summation, and tetanus; length–tension and force–velocity relationships; fatigue protocols; electromyography; methods for measuring intracellular calcium; biochemical, histological, and molecular analyses of muscle tissue; markers of oxidative stress and muscle damage; recording and analysis of experimental data; experimental design, quality control, and interpretation of scientific findings.
Objectives of the Course
The aim of this course is to teach, at the graduate level, basic and advanced laboratory methods used to investigate the physiological properties of skeletal, smooth, and cardiac muscle, and to develop students’ ability to plan experimental studies on muscle tissue, select appropriate methods, analyze experimental data, and interpret findings in relation to physiological mechanisms
Contribution of the Course to Field Teaching
Basic Vocational Courses X
Specialization / Field Courses X
Support Courses X
Transferable Skills Courses
Humanities, Communication and Management Skills Courses
Relationships between Course Learning Outcomes and Program Outcomes
Relationship Levels
Lowest Low Medium High Highest
1 2 3 4 5
# Program Learning Outcomes Level
P1 Evaluates theoretical and practical knowledge in sport management at an advanced level and adapts current approaches to the management processes of sport organizations. 1
P2 Integrates knowledge from management and sport sciences to analyze problems related to sport services and develop evidence-based solutions. 1
P3 Develops recommendations for strategic planning, human resources, financing, and marketing processes in sport organizations and evaluates these recommendations in relation to organizational objectives. 1
P4 Plans and evaluates sport events and projects in accordance with the principles of resource management, risk management, safety, accessibility, and sustainability. 1
P5 Uses knowledge of sport anatomy, exercise physiology, and cardiovascular physiology to explain responses to physical activity and exercise-induced adaptations. 3
P6 Integrates knowledge of metabolism, molecular physiology, and intercellular interactions to analyze the biological foundations of exercise and sport performance. 4
P7 Compares the purposes, applications, and limitations of exercise tests and physical activity assessment methods and interprets their results in relation to sport service planning. 4
P8 Uses the fundamental principles of training science to evaluate training programs in terms of training load, recovery, adaptation, and performance improvement. 1
P9 Evaluates the relationship between nutrition and exercise performance using knowledge of sport nutrition and metabolism and identifies the need for collaboration with nutrition professionals in sport services. 1
P10 Uses knowledge of sport psychology to analyze motivation, stress, communication, and team dynamics and develops management recommendations that support participation and psychological well-being in sport settings. 1
P11 Evaluates the fundamental principles of sport injury prevention and rehabilitation and develops recommendations for creating safe sport environments and coordinating relevant professionals. 1
P12 Designs sport services and projects that promote participation in physical activity and healthy living among groups with different ages and needs. 1
P13 Identifies a researchable problem in sport and independently plans and conducts a thesis study using appropriate research methods. 1
P14 Analyzes research data using appropriate statistical methods and critically interprets findings from laboratory and field research, taking methodological limitations into account. 1
P15 Conducts research and professional activities in accordance with the principles of scientific research and publication ethics, personal data protection, and professional responsibility. 1
P16 Follows scientific publications in the field in Turkish and at least one foreign language and communicates research findings and professional recommendations using academic writing, seminar, and presentation techniques. 1
Weekly Detailed Course Contents
Week Topics
1 Introduction to muscle physiology and basic principles of experimental muscle physiology
2 Anatomical and functional organization of muscle tissue; experimental sampling methods
3 Preparation of isolated skeletal muscle preparations and organ bath systems
4 Measurement of contractile force; isometric and isotonic contractions
5 Electrical stimulation methods, single muscle twitch, summation, and tetanus
6 Length–tension relationship and determination of optimal muscle length
7 Force–velocity relationship and experimental assessment of muscle mechanics
8 Muscle fatigue, recovery, and fatigue protocols
9 Electromyography (EMG) and assessment of muscle electrical activity
10 Methods for measuring intracellular Ca²⁺ in muscle tissue and investigation of excitation–contraction coupling
11 Assessment of energy metabolism, oxidative stress, and antioxidant systems in muscle tissue
12 Assessment of muscle damage and regeneration using biochemical and histopathological methods
13 Gene and protein expression analyses in muscle tissue; qPCR, Western blot, and ELISA applications
14 Experimental design, data analysis, interpretation of results, and discussion of current studies in muscle physiology
Textbook or Material
Resources Hall, J. E., & Hall, M. E. (2025). Guyton and Hall Textbook of Medical Physiology (15th ed.). Elsevier
ECTS / Working Load Table
Quantity Duration Total Work Load
Course Week Number and Time 3 14 42
Out-of-Class Study Time (Pre-study, Library, Reinforcement) 40 2 80
Midterms 0 0 0
Quiz 0 0 0
Homework 1 52 52
Practice 0 0 0
Laboratory 0 0 0
Project 0 0 0
Workshop 0 0 0
Presentation/Seminar Preparation 0 0 0
Fieldwork 0 0 0
Final Exam 1 52 52
Other 0 0 0
Total Work Load: 226
Total Work Load / 30 7,53
Course ECTS Credits: 8